This study was undertaken to investigate the cytotoxic activities of the petroleum ether extract of dietary oil against human rectum (HRT-18) and colon (HCT-48, HT-29) cancer cells in vitro, and sarcoma-180 cells in vivo.
Each cell line was cultured in medium containing serial concentrations of the cinnamon oil, bay oil, cod liver and linoleic acid for 72 hours. And than, the growth rate, dose-response, size distribution and morphological change of each cells were
determined, while the effects of petroleum ether extract of the cinnamon against the survival times of mice inoculated with S-180 cells were examined.
@ES The experimental results obtained are summarized as follows;
@EN 1. The doubling times of HCT-48, HRT-18 and HT-29 cells were 24, 22 and 22 hours, respectively.
2. The yields of the petroleum ether extract of cinnamon and cinnamon branch were 1.09% and 0.28%, respectively.
3. The growth inhibition rate of HCT-48, HRT-18 and HT-29 cells were gradually increased in proportion to the concentration of the cinnamon, bay, cod oil and linoleic acid in culture medium.
4. The cell size of HCT-48 incubated in culture medium containing the petroleum ether extract of cinnamon (20 g/ml) was significantly reduced, compared with that of the control group, and microscopic examination of the treated cells further
exhibited
distorted and shrunker shape.
5. Mice inoculated with Sarcoma-180 cells were shown to have prolonged survival time (about 33%) by the treatment of petroleum ether extract of cinnamon, compared with their control groups.
In view of the above result, it can be concluded that the petroleum ether extract of cinnamon showed strong cytotoxic activity against human rectum and colon cancer cell lines in vitro, and S-180 cells in vivo.
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